Direct measurement of collagen crosslinks with automatic amino acid analyzer - identification of peaks due to crosslinks
نویسندگان
چکیده
منابع مشابه
Quantitative determination of collagen crosslinks.
The primary functional role of collagen is as a supporting tissue and it is now established that the aggregated forms of the collagen monomers are stabilised to provide mechanical strength by a series of intermolecular cross-links. In order to understand the mechanical properties of collagen, it is necessary to identify and quantitatively determine the concentration of the cross-links during th...
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Reduction of insoluble collagens from bone, skin, and tendon with NsB3H4 produced several radioactive substances which were isolated from acid hydrolysates of the protein. One of these substances, aldol-histidine, was most abundant in cow skin collagen and also appeared in reconstituted fibrils of purified cow skin tropocollagen. Another compound, histidino-hydroxymerodesmosine, was abundant in...
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Lysyl oxidase catalyzes the formation of crosslinking aldehydes in collagen and elastin. This report demonstrates that the enzyme has high activity with collagen precipitated as native fibrils, an apparent K(m) of 0.95 muM, and low activity toward either soluble forms such as denatured collagen, isolated alpha chain, or isolated alpha1-CBl peptide, or precipitated collagen fibrils after pepsin ...
متن کاملThermal stability of DNA with interstrand crosslinks.
Although many anticancer drugs exert their biological activity by forming DNA interstrand crosslinks (ICLs), the thermodynamics of biologically relevant long crosslinked DNAs has not been intensively studied in contrast to short duplexes. Here, we carry out computer modeling of the shift of melting temperature of long DNAs caused by ICLs taking into account crosslinking effect in itself and con...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1976
ISSN: 0014-5793
DOI: 10.1016/0014-5793(76)80104-4